IOANNA KAKOULLI | Assistant Professor
Materials Science and Engineering
From macro to nano length scale imaging and analysis of bioarchaeological materials
This research focuses on the analysis of naturally mummified human remains from the Tarapacá Valley in northern Chile through investigations of the taphonomic and diagenetic processes of hair and skin and the study of the burial micro-environment. The innovation of this research is based the application of non-invasive and non-destructive methods of examination and analysis including field spectrophotometry, CT scanning, multispectral imaging spectroscopy, focused ion beam (FIB) milling and combined variable pressure scanning electron microscopy and Raman spectroscopy.
Scientific data from the examination of hair and skin specimens of different individuals within the same site showed discrete discrepancies in their morphology and structure. For example, Raman spectra of mummified skin from two different individuals indicated a significant marked loss of protein amide I (1680-1645 cm-1) and amide III (1305-1230 cm-1) on one of the samples analyzed.
The taphonomic histories involved at the Tarapacá valley, reflecting postdepositional processes seem to have transformed organic materials both physically and chemically. The occurred modifications in the chemistry and morphology of the materials analyzed have offered meaningful preliminary data to understand the preservation implications of the organic materials. Analyses of both hair and skin have shown promising results, supporting their potential use as taphonomic biomarkers, since variations within individual samples as a product of the diagenetic processes during burial – at a specific site in a given burial micro-environment – offered clues on site formation processes.
Ioanna Kakoulli, Assistant Professor, Materials Science and Engineering Department, Henry Samueli School of Engineering and Applied Science, with joint appointment in the UCLA/Getty Conservation Program, Cotsen Institute of Archaeology.
Professor Kakoulli began her research in archaeological and conservation sciences at the Courtauld Institute of Art, University of London and she continued her training in advanced analytical techniques applied in the archaeometric field at the University of Oxford from where she has received her Doctorate of Philosophy (D.Phil.) in Archaeological Sciences. She has acquired further scientific experience through internships and scientific collaborations at the Opificio delle Pietre Dure in Florence, the Scientific Laboratory of the J. Paul Getty Museum and the National Centre for Scientific Research “Demokritos” in Greece. She has participated actively in numerous archaeological excavations and field conservation projects and she has coordinated fieldwork and supervised undergraduate and graduate students. Through her studies and training, professor Kakoulli developed an expertise in the analytical procedures for micro-analysis and non-destructive methods using spectral imaging systems and variable pressure scanning electron microscopy and the advancement of field conservation during excavation and interdisciplinary approaches integrating science, conservation and archaeology. In 2005, Professor Kakoulli has created the Archaeomaterials Group aiming to establish a dedicated research group at UCLA to support research in archaeological and conservation science and forensic analyses of anthropological interest. Her current research projects involve forensic trace evidence studies using optical and molecular biopsies of archaeological human remains aiming to understand the detrimental role of the depositional burial environment and to explore the environmental factors associated with taphonomic processes; the spectroscopic study and provenance of materials; studies in field archaeological conservation techniques; and the development and application of non-invasive diagnostic techniques for the study of the materials and technology of archaeological and cultural artifacts. Professor Kakoulli is co-director of the Tarapacá Valley Archaeological Project in northern Chile and research collaborator in European and international programs. She is the author of various scientific articles in archaeological sciences and conservation and a member of national and international professional and scientific committees.